. 2018 Jun 21;13(6):e0198323. doi: 10.1371/journal.pone.0198323

Table 1. Clustering of the altered genes in response to TLR1/2 or TLR4 activation in SZ95 sebocytes.

Genes that were regulated both by PAM3CSK4 and LPS were functionally categorized using the Cytoscape classification system. The clusters clearly defined an immunocompetence for the activated sebocytes and pointed on so far unrevealed functions such as a possible involvement in wounding and leukocyte migration. Note that in black are the genes/clusters present in both the early (6 hours) as well as in the late responder (24 hours) group, while the genes in bold falling to the functional category of cholesterol metabolic process were only detected at 24 hours.

GO-ID	p-value	corr p-value	Description	Genes in test set
2376	1,46E-38	4,38E-35	immune system process	CXCL6\|CSF3\|CIITA\|CSF2\|CSF1\|SECTM1\|CXCL1\|CXCL3\|TNF\|CXCL2\| CTSS\|IFI44L\|CXCL5\|ICAM1\|OASL\|IFIH1\|C4B\|C4A\|KYNU\|DHX58\|C1RL\|TRIM25\|JUNB\|IKBKE\|CD34\|ICOSLG\|TRIM22\|SERPINB4\|EDN1\|IL4R\| IL1R1\|DDX58\|FST\|WNT5A\|TAP2\|TAP1\|TNFRSF1B\|HLA-F\|HLA-G\|MMP9\|TAPBP\|OAS1\|OAS2\|IL1B\|IRF1\|OAS3\|LCP1\|LTB\|S100A\|CFB\|LTF\|PTGER4\|SELPLG\|C1S\|C1R\|EBI3\|NOD2\|FYB\|RELB\|C3\|SBNO2\|EBP\|IFI16\|NFIL3\|IRAK2\|CCL5\|UBD\|CCL2\|APOL1\|GBP2\|TNFSF18\|IL32\| CD74\|TGFB2\|VCAM1\|CCL20\|INHBA\|SOD2\|PML\|NFKB2\|BST2\|NFKBI\| CXCL10\|CXCL11\|IL6\|VNN1\|BCL6\|IL8\|BCL3\|LCN2\|SAA1\|TNFSF9\|SAA\|PTX3\|IL7R
6955	4,97E-32	7,47E-29	immune response	CXCL6\|CSF3\|CIITA\|CSF2\|SECTM1\|CXCL1\|CXCL3\|TNF\|CXCL2\|CTSS\| IFI44L\|CXCL5\|ICAM1\|OASL\|IFIH1\|C4B\|C4A\|KYNU\|DHX58\|C1RL\| TRIM25\|IKBKE\|TRIM22\|SERPINB4\|IL4R\|IL1R1\|DDX58\|WNT5A\| TNFRSF1B\|HLA-F\|HLA-G\|TAPBP\|OAS1\|OAS2\|IL1B\|OAS3\|LCP1\|LTB\|CFB\|LTF\|PTGER4\|C1S\| C1R\|EBI3\|NOD2\|FYB\|RELB\|C3\|SBNO2\|NFIL3\|CCL5\|UBD\|CCL2\| APOL1\|GBP2\|TNFSF18\|IL32\|CD74\|CCL20\|NFKB2\|BST2\|CXCL10\| CXCL11\|IL6\|VNN1\|IL8\|BCL3\|LCN2\|TNFSF9\|PTX3\|IL7R
6952	3,98E-31	3,99E-28	defense response	CXCL6\|CIITA\|SERPINE1\|CXCL1\|CXCL3\|TNF\|CXCL2\|IFIH1\|C4B\|C4A\| KYNU\|DHX58\|C1RL\|NFKBIZ\|BDKRB2\|OLR1\|TRIM25\|AOX1\|ICOSLG\| IL1R1\|DDX58\|WNT5A\|PLA2G4C\|TAP1\|F3\|HLA-G\|ADORA2A\|ELF3\|IL1B\|LAP3\|S100A9\|CFB\|S100A8\|IDO1\|LTF\|SAA3P\|C1S\|C1R\|SAA4\|NOD2\|C3\|IRAK2\|CCL5\|UBD\|CCL2\|APOL1\|APOL3\|IL32\|CD74\|VCAM1\|KLRC2\|CCL20\|MX2\|STAT3\|MX1\|INHBA\|NFKB1\|ASS1\|FOSL1\|CXCL10\|CXCL11\|IL6\|VNN1\|IL8\|TNIP1\|BCL3\|LCN2\|SAA1\|SAA2\|PTX3
9611	1,60E-24	9,60E-22	response to wounding	NRP1\|CXCL6\|CIITA\|SERPINE1\|ADM\|CXCL1\|CXCL3\|TNF\|CXCL2\|C4B\| THBD\|C4A\|PLAU\|C1RL\|NFKBIZ\|BDKRB2\|OLR1\|AOX1\|IFNGR1\| WNT5A\|PLA2G4C\|F3\|EREG\|PLSCR1\|ADORA2A\|LOX\|ELF3\|IL1B\|LAP3\|S100A9\|CFB\|S100A8\|IDO1\|SAA3P\|C1S\|C1R\|SAA4\|TFPI\|C3\|IRAK2\| CCL5\|CCL2\|APOL3\|TGFB2\|VCAM1\|CCL20\|STAT3\|SOD2\|NFKB1\|ASS1\|CXCL10\|CXCL11\|IL6\|VNN1\|IL8\|SAA1\|SAA2\|PTX3
6954	3,27E-24	1,64E-21	inflammatory response	SAA3P\|CXCL6\|CIITA\|C1S\|C1R\|SAA4\|CXCL1\|CXCL3\|TNF\|CXCL2\|C3\| C4B\|C4A\|IRAK2\|CCL5\|C1RL\|NFKBIZ\|BDKRB2\|OLR1\|CCL2\|AOX1\| APOL3\|VCAM1\|CCL20\|STAT3\|PLA2G4C\|F3\|NFKB1\|ASS1\|CXCL10\| CXCL11\|IL6\|VNN1\|ADORA2A\|IL8\|ELF3\|IL1B\|SAA1\|SAA2\|PTX3\|LAP3\|S100A9\|CFB\|S100A8\|IDO1
6950	1,34E-22	5,74E-20	response to stress	SERPINE1\|TNF\|C4B\|C4A\|PLAU\|DHX58\|C1RL\|TRIM25\|ICOSLG\|IL1R1\|WNT5A\|DIO2\|TAP1\|HLA-G\|EREG\|LOX\|LAP3\|TRIB1\|S100A9\|CFB\|S100A8\|IDO1\|INSIG1\|ADRB2\|C3\|SOCS3\|IRAK2\|UBD\|JUN\|TGFB2\|VCAM1\|KLRC2\|DHCR24\|INHBA\| NFKB1\|FOSL1\|CXCL10\|CXCL11\|IL6\|VNN1\|BCL6\|IL8\|BCL3\|MAFF\| LCN2\|NRP1\|CXCL6\|CIITA\|PCSK9\|ADM\|CXCL1\|CXCL3\|CXCL2\|HK2\| IFIH1\|THBD\|DUSP10\|UCN2\|KYNU\|NFKBIZ\|BDKRB2\|OLR1\|AOX1\| IKBKE\|EDN1\|IFNGR1\|DDX58\|PLA2G4C\|F3\|PLSCR1\|ADORA2A\|ELF3\| IL1B\|ALDOC\|SGK1\|LTF\|SAA3P\|C1S\|C1R\|SAA4\|NOD2\|PTGS2\|TFPI\| IFI16\|CCL5\|CCL2\|APOL1\|APOL3\|IL32\|ARNT2\|CD74\|CCL20\|STAT1\| MX2\|STAT3\|MX1\|SOD2\|AGT\|ASS1\|PML\|TNIP1\|SAA1\|SAA2\|PTX3
42127	5,99E-17	1,20E-14	regulation of cell proliferation	NRP1\|CSF3\|IFITM1\|CSF2\|CSF1\|SERPINE1\|PTPRO\|ADM\|CXCL1\|TNF\| CXCL5\|CDH5\|PLAU\|MYC\|NAMPT\|BDKRB2\|ICOSLG\|TGM2\|FGFBP1\| EDN1\|SP110\|IGFBP3\|WNT5A\|SSTR1\|F3\|RUNX3\|EREG\|ADORA2A\| IL1B\|MVD\|TRIB1\|IDO1\|EBI3\|ADRB2\|PTGS2\|CCL2\|ARNT2\|CD74\|JUN\|TGFB2\|VCAM1\|STAT1\|TNFRSF9\|IL34\|RARRES1\|VEGFC\|DHCR24\| INHBA\|SOD2\|AGT\|PML\|FOSL2\|FOSL1\| NFKBIA\|CXCL10\|IL6\|BCL6\|FAP\|IL8\|TNFSF9\|DHCR7
8203	3,55E-16	6,67E-14	cholesterol metabolic process	IDI1\|FDPS\|MVK\|HMGCS1\|INSIG1\|CYP51A1\|PCSK9\|DHCR24\| HMGCR\|LSS\|ACAT2\|SQLE\|NSDHL\|EBP\|FAP\|MVD\|DHCR7\|APOL1\| LDLR\|APOL3\|FDFT1
2526	6,81E-13	7,58E-11	acute inflammatory response	SAA3P\|VCAM1\|C1S\|C1R\|STAT3\|SAA4\|F3\|ASS1\|C3\|C4B\|C4A\|IL6\| VNN1\|C1RL\|SAA1\|SAA2\|CFB\|APOL3
50900	3,99E-12	3,99E-10	leukocyte migration	TGFB2\|SELPLG\|VCAM1\|CXCL3\|TNF\|ICAM1\|IL6\|IL8\|CCL5\|IL1B\|SAA1\|CCL2\|SAA2\|S100A9\|CD34