Table 1. Characteristics of gametocyte carriers and mosquito feeding assays.
| Ouelessebougou, Mali | Bobo Dioulasso, Burkina Faso |
Balonghin, Burkina Faso |
Yaoundé, Cameroon |
|
|---|---|---|---|---|
| Number of experiments | 71 | 19 | 45 | 13 |
| Enrolment criteria: | Detection of gametocytes by microscopy | Detection of gametocytes by microscopy | Detection of gametocytes by molecular QT-NASBA | Detection of gametocytes by microscopy |
| Period and season of data collection | January 2013-November 2014 (dry and wet season) | April-June 2016 (dry season) | October-November 2014 (wet season) | October-December 2015 (wet season) |
| P. falciparum parasite prevalence in cross-sectional surveys in the study area (microscopy) | 70.2% in children < 5 years, 2015–16 (Mahamar et al., 2017) | 40.9–61.7% in children 1–9 years, 2014–2015 (Hien et al., 2017) | 59.7% in children < 15 years, 2014 wet season (Gonçalves et al., 2017) | 44.7–55.6% in children 4–15 years, 2013–2014 (Sandeu et al., 2017) |
| Age, median (IRQ) | 11 (7–25) | 5–15 (range)* | 10 (8–13) | 9 (6–11) |
| Asexual parasite prevalence % (n/N) | 64.8 (46/71) | 73.7 (14/19) | 73.3 (33/45) | 76.9 (10/13) |
| Asexual parasite density per µL, median (IQR) | 432 (96–2880) | 360 (240–1040) | 658 (336–1237) | 944 (288–4224) |
| Total gametocyte density per µL, median (IQR) | 62.8 (31.4–146.8) | 19.2 (10.5–26.1) | 4.0 (0.6–11.0) | 64.4 (11.7–126.2) |
| Percentage of male gametocytes, median (IQR) | 14% (7–25%) | 51% (39–66%) | 30% (18–40%) | 32% (27–53%) |
| Number of mosquitoes examined per experiment, median (IQR) | 70 (63–79) | 29 (28–30) | 40 (35–45) | 37 (32–45) |
| Infectious individuals, % (n/N) | 74.7 (53/71) | 84.2 (16/19) | 22.2 (10/45) | 76.9 (10/13) |
| Infected mosquitoes, % (n/N) | 17.0 (842/4960) | 39.2 (208/531) | 3.5 (63/1783) | 38.1 (184/483) |
*the age of individual gametocyte donors was not recorded in Bobo Dioulasso; gametocyte carriers were recruited from the age range 5–15 years; asexual parasite density was determined by microscopy, gametocyte density by quantitative reverse transcriptase PCR. QT-NASBA = Pfs25 mRNA quantitative nucleic acid sequence based amplification.