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. 2018 Jun 12;7:e33246. doi: 10.7554/eLife.33246

Figure 4. Nicotinamide riboside is incorporated intact into mitochondrial NAD.

Figure 4.

(A) Isotopically-labeled nicotinamide riboside (NR) or nicotinic acid (NAR) was synthesized to contain a C-13 on the pyridine carboxyl group and a deuterium on the ribose moiety (NAR labeling shown). (B) Fractional labeling of NAD in C2C12 whole cell lysate and isolated mitochondria following 4 hr of incubation with double-labeled NR with or without the NMNAT inhibitor, gallotannin (Gallo; 100 µM). (No label group, N = 1; NR 4 hr group, N = 4; NR + Gallo 4 hr group, N = 3). (C) Fractional labeling of NMN found in C2C12 whole cell lysate and isolated mitochondria following 4 hr of incubation with double-labeled NR with or without gallotannin (Gallo; 100 µM). (No label group, N = 1; NR 4 hr group, N = 4; NR + Gallo 4 hr group, N = 3). Data shown are means ± SEM and are representative of 2 independent experiments.