Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Cell line (Mus musculus)	C2C12; myotubes; myoblasts;	ATCC	ATCC CRL-1772	Mouse myoblast, mycoplasma negative
Cell line (Homo sapiens)	HL-60	ATCC	ATCC CCL-240	Human leukemia, authenticated by STR profiling, mycoplasma negative
Cell line (Homo sapiens)	293; HEK293	Gift from Morris Birnbaum’s lab		Human embryonic kidney, authenticated by STR profiling, mycoplasma negative
Strain, strain background (Mus musculus)	C57BL/6 mice	The Jackson Laboratory	000664	C57BL/6J
Recombinant DNA reagent	LentiCRISPR v2 (Lentiviral vector)	Addgene	52961
Recombinant DNA reagent	psPAX2 (Lentiviral packaging plasmid)	Addgene	12260
Recombinant DNA reagent	pMD2.G (Lentiviral envelope expressing plasmid)	Addgene	12259
Recombinant DNA reagent	pLX304 (Gateway Lentiviral vector)	DNASU plasmid repository	NMNAT1	Clone ID: HsCD00434593
Chemical compound, drug	ATP	Sigma-Aldrich	A2383
Chemical compound, drug	Protease	Sigma-Aldrich	P5380	Protease from Bacillus lichenformis
Chemical compound, drug	ADP	Sigma-Aldrich	A2754
Chemical compound, drug	Pyruvate	Sigma-Aldrich	P2255
Chemical compound, drug	Malate	Sigma-Aldrich	M1000
Chemical compound, drug	B-NMN	Sigma-Aldrich	N3501 100 MG
Chemical compound, drug	PRPP	Sigma-Aldrich	P8296
Chemical compound, drug	FCCP	Sigma-Aldrich	C2920-10MG
Chemical compound, drug	Oligomycin	Sigma-Aldrich	O4876-5MG
Chemical compound, drug	Protease inhibitor cocktail (Sigma); PI	Sigma-Aldrich	P8340	Protease inhibitor cocktail solution
Chemical compound, drug	Alcohol dehydrogenase	Sigma-Aldrich	A3263-150KU
Chemical compound, drug	Diaphorase	Sigma-Aldrich	D5540-500UN
Chemical compound, drug	Resazurin	Sigma-Aldrich	R7017
Chemical compound, drug	Flavin mononucleotide	Sigma-Aldrich	F6750
Chemical compound, drug	Nicotinamide; NAM	Sigma-Aldrich	72345
Chemical compound, drug	Hexadinitrine	Sigma-Aldrich	107689
Chemical compound, drug	Nicotinic acid; NA	Sigma-Aldrich	N4126
Chemical compound, drug	Perchloric Acid	Sigma-Aldrich	244252
Chemical compound, drug	NAD; Nicotinamide adenine dinucleotide	Roche	101127965001
Chemical compound, drug	Proteinase K; ProtK	Roche	03115887001
Chemical compound, drug	Protease inhibitor cocktail (Roche)	Roche	11697498001	Complete protease inhibitor cocktail tablets
Chemical compound, drug	BSA	Roche	03117057001	Bovine serum albumin Fraction V, heat shock, fatty acid free
Chemical compound, drug	Puromycin	ThermoFisher Scientific	A111380-03	10 mg/mL stock
Chemical compound, drug	Blasticidin	ThermoFisher Scientific	R21001
Chemical compound, drug	Gallotannin	Enzo Life Sciences	ALX-270–418 G001
Chemical compound, drug	Insulin	Novo-Nordisk Novolin N	U-100	100 units/mL; recombinant DNA origin
Chemical compound, drug	Fugene 6	Promega	E2691
Chemical compound, drug	NR; isotope-labeled nicotinamide riboside	PMID: 27508874
Chemical compound, drug	NAR; isotope-labeled nicotinic acid riboside	this paper
Commercial assay or kit	Micro BCA Protein Assay Kit	Thermo Fisher Scientific	23235
Commercial assay or kit	SuperSignal West femto kit	Thermo Fisher Scientific	34095
Antibody	anti-NMNAT1 (rabbit polyclonal)	Gift from Lee Kraus, Zhang et al. (2009) PMID: 19478080
Antibody	anti-VDAC (rabbit monoclonal)	Abcam	ab154856	[EPR10852(B)]
Antibody	anti-B-actin HRP (mouse monoclonal)	Abcam	ab20272	[mAbcam 8226]
Antibody	Secondary antibody	GE Healthcare Life Sciences	NA934	Amersham ECL anti-rabbit IgG, HRP-linked whole Ab (from donkey)
Antibody	Secondary antibody	GE Healthcare Life Sciences	NA931	Amersham ECL anti-mouse IgG, HRP-linked whole Ab (from sheep)