Figure 4. Bloodlinc Localizes to trans-Chromosomal Loci Encoding Key Erythropoiesis Modulators.

(A) Bloodlinc genomic occupancy is enriched in genic regions.

(B) Bloodlinc occupancy is focal, specific, and can occur within regulatory regions distal to erythroid-active genes, such as the Klf1/Dnase2a locus. Top tracks display signal density for Bloodlinc ChIRP-seq with all probes or with split probe pools, as well as input, RNase-treated, and no probe controls. Bottom tracks display strand-specific maps of poly(A)⁺ RNA and poly(A)⁻ RNA signal density, and occupancy maps of RNA polymerase II, H3K4me3, H3K4me1, and H3K27ac. Gene models are depicted in blue.

(C) Bloodlinc diffuses from its transcription site to trans-chromosomal gene targets that are reciprocally regulated upon Bloodlinc knockdown and overexpression. Tracks show expression changes (log₂ fold change over control) of 81 genes differentially and reciprocally regulated upon Bloodlinc shRNA-mediated knockdown (KD; outer tracks) or ectopic overexpression (OV; inner tracks), inscribed at their respective genomic locales. Depletion data are pooled from two separate shRNAs. The center of the circle depicts Bloodlinc diffusion to its ChIRP-seq occupancy sites as directional links from its genomic locus. Links to occupancy sites intersecting genic regions are highlighted in red.

See also Figure S6 and Table S3.