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. Author manuscript; available in PMC: 2018 Jun 21.
Published in final edited form as: Cell Rep. 2017 Jun 20;19(12):2503–2514. doi: 10.1016/j.celrep.2017.05.082

Figure 6. Bloodlinc Can Promote Terminal Red Cell Development.

Figure 6

(A) Ectopically expressed Bloodlinc promotes proliferation of erythroid precursors. Shown are live cell numbers of primary erythroid progenitors transduced with empty or Bloodlinc-expressing vector and kept in maintenance medium.

(B) Bloodlinc ectopic expression stimulates erythroid precursor cell cycling. Cells in G1 or in S/G2/M phases after culture for 48 hr in maintenance medium are quantified.

(C) Ectopically expressed Bloodlinc prompts erythroid precursors to terminally differentiate and undergo enucleation. Flow cytometry plots show levels of the differentiation marker TER119 versus DNA content for live primary erythroid progenitors cultured for 48 hr in maintenance medium. Lower right quadrants identify enucleated reticulocytes, quantified to the right.

Data are mean ± SEM from n = 2 experiments with n = 1 or 2 replicate measurements each. *p < 0.05 relative to control, t test. See also Figure S6.