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. Author manuscript; available in PMC: 2019 Jan 12.
Published in final edited form as: ACS Infect Dis. 2017 Nov 10;4(1):46–52. doi: 10.1021/acsinfecdis.7b00139

Figure 1. Overview of the two-dimensional human small intestine (HSI) monolayer utilized.

Figure 1

(A) Schematic of the Transwell apparatus containing a collagen scaffold, upon which human small intestinal monolayers are cultured. (B) Longitudinal cross section of monolayers stained with F-actin and Integrin β4 to label the luminal and basolateral regions, respectively. (C) En face view of monolayers stained with F-actin and E-cadherin reveals characteristic flagstone staining pattern. Scale bar, 25 μm. (D) HSI monolayers consist primarily of proliferating, EdU+ cells. Colors used: EdU, red; DNA, blue.