Skip to main content
. Author manuscript; available in PMC: 2019 Jan 12.
Published in final edited form as: ACS Infect Dis. 2017 Nov 10;4(1):46–52. doi: 10.1021/acsinfecdis.7b00139

Figure 2. Diclofenac exerts cytotoxicity by reducing mitochondrial membranepotential and inducing O2•−.

Figure 2

(A) DCF-treated cells have reduced Mitotracker CMH2-XRos staining compared to vehicle-treated control. Images were acquired at 20x and are representative images from three independent experiments. (B) Quantification of the mean fluorescence intensity of (A) demonstrates that DCF significantly reduces CMH2-XRos staining. (C) Compared to untreated control, 24h treatment with DCF exerts significant dose-dependent cytotoxicity, as measured by CellTox Green endpoint assay. ** p<0.01 by one-way ANOVA with Dunnett’s test for multiple comparisons to the control. (D) MitoSOX staining reveals high levels of O2•− generated in response to DCF, within 24h. Cells are counterstained in both (A) and (D) with Hoechst to label nuclei, and Mitotracker Green to label mitochondria. Scale bar, 25 μm.