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. Author manuscript; available in PMC: 2018 Dec 21.
Published in final edited form as: Mol Cell. 2017 Dec 7;68(6):1108–1119.e3. doi: 10.1016/j.molcel.2017.11.008

Figure 3. Spatial Separation of CDC20 and CYCB mRNAs in Prophase Cells.

Figure 3

(A) Co-expression of CDC20 with cell cycle genes as revealed by double RNA FISH coupled with DAPI staining.

(B) CDC20 does not co-express with an S-phase expressed gene HIS4. CDC20 and cell cycle genes were detected by gene specific probes with different labelling. Scale bars in (A) and (B), SAM overview (top panels) = 50 µm; single cells (bottom panels) = 5 µm.

(C) Quantification of the number of cells that express CDC20 and CYCB genes at different mitotic stages. CYCB1 genes were mostly expressed at prophase and metaphase, and largely co-express with CDC20.

See also Figure S3.