(A) Co-expression of CDC20 with cell cycle genes as revealed by double RNA FISH coupled with DAPI staining.
(B) CDC20 does not co-express with an S-phase expressed gene HIS4. CDC20 and cell cycle genes were detected by gene specific probes with different labelling. Scale bars in (A) and (B), SAM overview (top panels) = 50 µm; single cells (bottom panels) = 5 µm.
(C) Quantification of the number of cells that express CDC20 and CYCB genes at different mitotic stages. CYCB1 genes were mostly expressed at prophase and metaphase, and largely co-express with CDC20.
See also Figure S3.