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. Author manuscript; available in PMC: 2018 Dec 21.
Published in final edited form as: Mol Cell. 2017 Dec 7;68(6):1108–1119.e3. doi: 10.1016/j.molcel.2017.11.008

Figure 5. CDC20 5’UTR Is Involved in mRNA Nuclear Localization.

Figure 5

(A) Schematic diagram of CDC20 mRNA deletion constructs.

(B) Quantification of the number of prophase cells expressing GFP fused CDC20 mRNAs that contain serial deletions. CYCB1;2 expression was used as a prophase marker. All GFP-CDC20 mRNAs with deletions in the CDC20 ORF were found to localize in the nucleus. Each pair of columns represents data from one meristem.

(C) Localization of GFP-CDC20 truncated mRNAs lacking CDC20 5’UTR or 3’UTR. Deletion of 5’UTR abolished GFP-CDC20 mRNA nuclear sequestration, leading to nucleocytoplasmic or mostly cytoplasmic localization. Scale bars, 50 µm for SAM overview (top panels) and 5 µm for single cells (bottom panels).

(D) Quantification of the number of prophase cells expressing full length, 3’UTR deleted, and 5’UTR deleted GFP-CDC20 mRNAs. Each pair of columns represents data from one meristem.

See also Figure S5.