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. Author manuscript; available in PMC: 2018 Dec 21.
Published in final edited form as: Mol Cell. 2017 Dec 7;68(6):1108–1119.e3. doi: 10.1016/j.molcel.2017.11.008

Figure 6. Dual Roles of 5’UTR in CDC20 mRNA Nuclear Localization and Translation.

Figure 6

(A) Schematic diagram of chimeric mRNA construction in which GFP was fused with CDC20 5’UTR. GFP alone was used a control.

(B) Localization of 5’UTRCDC20-GFP and GFP mRNAs in prophase cells. Scale bars, 50 µm for SAM overview (top panels) and 5 µm for single cells (bottom panels).

(C) Quantification of the number of prophase cells expressing5’UTRCDC20-GFP and GFP mRNAs. Each pair of columns represents cell numbers from one meristem.

(D) The expression of GFP-CDC20 fusion protein in root and SAM as revealed. No GFP fluorescence could be observed in 5’UTR truncated GFP-CDC20 transgenic plants. Scale bar, 50 µm.

(E) The number of transgenic lines analysed. GFP-CDC20 expression was detected in 22/23 lines of full length GFP-CDC20 plants, 15/21 lines of 3’UTR truncated GFP-CDC20 transgenic plants, and 0/23 of 5’UTR truncated GFP-CDC20 transgenic plants.

See also Figure S5.