(A) Schematic diagram of chimeric mRNA construction in which GFP was fused with CDC20 5’UTR. GFP alone was used a control.
(B) Localization of 5’UTRCDC20-GFP and GFP mRNAs in prophase cells. Scale bars, 50 µm for SAM overview (top panels) and 5 µm for single cells (bottom panels).
(C) Quantification of the number of prophase cells expressing5’UTRCDC20-GFP and GFP mRNAs. Each pair of columns represents cell numbers from one meristem.
(D) The expression of GFP-CDC20 fusion protein in root and SAM as revealed. No GFP fluorescence could be observed in 5’UTR truncated GFP-CDC20 transgenic plants. Scale bar, 50 µm.
(E) The number of transgenic lines analysed. GFP-CDC20 expression was detected in 22/23 lines of full length GFP-CDC20 plants, 15/21 lines of 3’UTR truncated GFP-CDC20 transgenic plants, and 0/23 of 5’UTR truncated GFP-CDC20 transgenic plants.
See also Figure S5.