Figure 1.

In Vivo Immediate-Early Gene Imaging

(A–C) Regions of interest (ROIs) selected for 2-photon imaging. (A) Representation of the top view of the mouse brain based on the Allen Institute’s 3D mouse brain atlas with the positions of the ROIs. The dorsal (dysgranular) retrosplenial cortex (RSC) is highlighted in yellow. (B) A zoomed-in image showing the RSC. The images in (C) are brightfield photographs of the craniotomy areas in each animal. The numbering of cases and associated color-code is carried forward to subsequent figures.

(D) Task-evoked change in fluorescence. The white outlines denote the positions of cells detected across the whole study, while the coloring indicates the level of fluorescence observed at four timepoints following exposure to the maze on a single session. The intervals are 1.5–2 hr (T1), 2–2.5 hr (T2), 2.5–3 hr (T3), and 3–3.5 hr (T4) from the beginning of the task, respectively.

See also Figure S1.