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. 2018 Jun 18;28(12):1975–1980.e6. doi: 10.1016/j.cub.2018.05.002
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit polyclonal anti-c-fos Synaptic Systems GmBH Cat. #226 003
Goat anti-rabbit AlexaFluor-405-IgG Abcam ab175652
Rabbit monoclonal anti-NeuN conjugated with Alexa Fluor 647 Abcam ab190565
Rabbit polyclonal anti-GFP-Alexa Fluor 488 Fisher Scientific Cat. #A-21311

Chemicals, Peptides, and Recombinant Proteins

Euthathal Merial Animal Health N/A (provided by vet)
Dexadreson (dexamethasone) Intervet UK Ltd N/A (provided by vet)
Metacam (meloxicam) Boehringer Ingelheim N/A (provided by vet)
Chloramphenicol 1% Martindale Pharmaceuticals Ltd N/A (provided by vet)
Germolene Bayer Available as over the counter product
Lidocaine hydrochloride 2% Pfizer N/A (provided by vet)
Vetbond 3M 1469
Super-Bond C&B Sun Medical N/A
3 mm small round cover glass Harvard Apparatus Ltd 64-0720
Sodium chloride Merck 1064041000
Sodium hydrogen carbonate Merck 1063291000
Potassium chloride Merck 1049361000
Sodium dihydrogen phosphate monohydrate Merck 1063461000
Magnesium chloride hexahydrite Merck 1058331000
D(+)-glucose Merck 1083371000
Oxoid™ phosphate buffered saline tablets Fisher Scientific BR0014G
Triton-X Merck T8787-50ML
Paraformaldehyde Merck P6148-1KG
Sucrose Merck S9378-1KG
Goat serum Merck G9023-5ML
Fluoromount Aqueous Mounting Medium Merck F4680-25ML
Strawberry milk Yazoo, FrieslandCampina N/A
Vanilla and lemon extracts FoodCare N/A

Experimental Models: Organisms/Strains

B6.Cg-Tg(Fos-tTA,Fos-EGFP)1Mmay/J mouse The Jackson Laboratory RRID: IMSR_JAX:018306

Software and Algorithms

Fiji (ImageJ) [42] https://fiji.sc
ImageJ StackReg plugin [43] http://bigwww.epfl.ch/thevenaz/stackreg/
ImageJ Template Matching plugin [44] https://sites.google.com/site/qingzongtseng/template-matching-ij-plugin
ImageJ bUnwarpJ plugin [45] https://imagej.net/BUnwarpJ
Custom ImageJ macros This paper Mendeley Data: https://doi.org/10.17632/45ts33k2tz.1
R R Project Version 3.4.3
https://www.r-project.org/
RStudio https://www.rstudio.com/ Version 1.1.383
R code This paper Mendeley Data: https://doi.org/10.17632/45ts33k2tz.1
SPSS IBM Version 23