REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rabbit polyclonal anti-c-fos | Synaptic Systems GmBH | Cat. #226 003 |
Goat anti-rabbit AlexaFluor-405-IgG | Abcam | ab175652 |
Rabbit monoclonal anti-NeuN conjugated with Alexa Fluor 647 | Abcam | ab190565 |
Rabbit polyclonal anti-GFP-Alexa Fluor 488 | Fisher Scientific | Cat. #A-21311 |
Chemicals, Peptides, and Recombinant Proteins | ||
Euthathal | Merial Animal Health | N/A (provided by vet) |
Dexadreson (dexamethasone) | Intervet UK Ltd | N/A (provided by vet) |
Metacam (meloxicam) | Boehringer Ingelheim | N/A (provided by vet) |
Chloramphenicol 1% | Martindale Pharmaceuticals Ltd | N/A (provided by vet) |
Germolene | Bayer | Available as over the counter product |
Lidocaine hydrochloride 2% | Pfizer | N/A (provided by vet) |
Vetbond | 3M | 1469 |
Super-Bond C&B | Sun Medical | N/A |
3 mm small round cover glass | Harvard Apparatus Ltd | 64-0720 |
Sodium chloride | Merck | 1064041000 |
Sodium hydrogen carbonate | Merck | 1063291000 |
Potassium chloride | Merck | 1049361000 |
Sodium dihydrogen phosphate monohydrate | Merck | 1063461000 |
Magnesium chloride hexahydrite | Merck | 1058331000 |
D(+)-glucose | Merck | 1083371000 |
Oxoid™ phosphate buffered saline tablets | Fisher Scientific | BR0014G |
Triton-X | Merck | T8787-50ML |
Paraformaldehyde | Merck | P6148-1KG |
Sucrose | Merck | S9378-1KG |
Goat serum | Merck | G9023-5ML |
Fluoromount Aqueous Mounting Medium | Merck | F4680-25ML |
Strawberry milk | Yazoo, FrieslandCampina | N/A |
Vanilla and lemon extracts | FoodCare | N/A |
Experimental Models: Organisms/Strains | ||
B6.Cg-Tg(Fos-tTA,Fos-EGFP∗)1Mmay/J mouse | The Jackson Laboratory | RRID: IMSR_JAX:018306 |
Software and Algorithms | ||
Fiji (ImageJ) | [42] | https://fiji.sc |
ImageJ StackReg plugin | [43] | http://bigwww.epfl.ch/thevenaz/stackreg/ |
ImageJ Template Matching plugin | [44] | https://sites.google.com/site/qingzongtseng/template-matching-ij-plugin |
ImageJ bUnwarpJ plugin | [45] | https://imagej.net/BUnwarpJ |
Custom ImageJ macros | This paper | Mendeley Data: https://doi.org/10.17632/45ts33k2tz.1 |
R | R Project | Version 3.4.3 https://www.r-project.org/ |
RStudio | https://www.rstudio.com/ | Version 1.1.383 |
R code | This paper | Mendeley Data: https://doi.org/10.17632/45ts33k2tz.1 |
SPSS | IBM | Version 23 |