Figure 4.

Cell Size Measurements by Quantitative Microscopy during the Coenocytic Growth

(A) Boxplots of cell volume measurements of DAPI-stained fixed cells. For 8-, 16-, 32-, and 2-nuclei cells, n > 67, 95, 21, and13, respectively.

(B) Boxplots of number of nuclei-to-volume ratio of DAPI-stained cells (n > 55 for each population).

(C) Representative images of cells with the same number of nuclei grown in 1× MB or 1/16× MB. Scale bar, 10 μm.

(D) Relative cell area, normalized to area at 0 hr, obtained from time-lapse live imaging of S. arctica in different media concentrations. n = 31, 52, 37, and 55 for 1×, 1/2×, 1/4×, and 1/16× media, respectively.

(E) Boxplots of relative cell area of live-imaged cells at 36 hr, grown in different media concentration. P values for Wilcoxon rank sum test.

(F) Representative kymographs of cells during time-lapse imaging of S. arctica in different media concentration. Scale bar, 10 μm.

(G) A schematic representation of the relationship between nuclear division cycles and cytoplasmic volume growth of S. arctica in various conditions. In lower nutrient concentration, the timing of nuclear cycles remains the same, but the cell volume grows more slowly.

See also Video S1.