Fig. 5.

T-tubular structure was preserved and SR Ca²⁺ released synchronised by BB. A Binary images of t-tubule morphology (top) and following skeletonization (bottom). B The regularity of t-tubules (fast Fourier transform power) was reduced in MCT compared to CON but preserved by BB treatment. Myocyte number given in the fig. C Tubule orientation was mainly transverse in CON and MCT + BB myocytes but more longitudinal in MCT myocytes. D Representative Western blots of BIN-1, JP2 and GAPDH. Note difference in group orders between blots and data, protein ladders separate group samples for BIN-1. E Protein levels of BIN-1 were reduced in MCT and MCT + BB. F JP2 was not different between groups. G Confocal linescan recordings show spatial non-uniformities in Ca²⁺ transients from MCT cells. H Ca²⁺ release was more heterogeneous in MCT cells. I Mean time to peak Ca²⁺ was slower in MCT. B,C, N = 3–4 rats per group, E,F N = 6 rats per group, H,I,N = 5–8 rats per group. B,C,F,H,I one-way ANOVA, E non-parametric ANOVA. *P < .05.