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A) The zeta potential of DPPC liposomes becomes more negative as the fraction of negatively-charged DPPG (inset) increases. However, partitioning of MPPC lysolipid into the membrane is independent of the zeta potential. B) Deprotonation of the terminal amine group (inset) of DPPE at pH 9 leads to a decrease in zeta potential for 5:95 DPPE:DPPC liposomes. However, the surface charge did not impact MPPC lysolipid partitioning into the membrane.
