(A) SV2C is expressed in the substantia nigra pars compacta (SNc) and pars reticulata (SNr). (B) We observed light SV2C immunoreactivity in the somata of neuromelanin-containing SNc cells, which is contrasted to the lack of immunoreactivity in an unstained SNc section. Neuromelanin is visible in the unstained tissue (C). (D) SV2C is expressed in the somata and neuropil of the putamen (Pu) and, to a lesser extent, the caudate nucleus (Cd, E). We also observed immunoreactivity in the somata within the globus pallidus (GP, F). (G) SV2C is highly colocalized with the vesicular GABA transporter (VGAT) within the putamen. Scale bars = 500 µm (A, D–F), 20 µm (C–B, D–E insets, G).