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. Author manuscript; available in PMC: 2018 Jun 22.
Published in final edited form as: ACS Chem Neurosci. 2018 Mar 23;9(6):1269–1275. doi: 10.1021/acschemneuro.8b00079

Table 1.

Immunoconjugate Formulation Parameters and Vaccine Schedule Used for Each Groupa

Vaccination Group Immunoconjugate (μg/dose) Conjugation Number Alum (mg/mL) Adjuvant Antinoc. Drug BBB Drug
Fent-KLH 50 μg Fent-KLH 11.8 0.8 50 μg CpG Fentanyl Fentanyl + Heroin
Her-KLH 50 μg Her-KLH 7.3 0.8 50 μg CpG Heroin Fentanyl + Heroin
Fent-KLH + Her-KLH 25 μg Fent-KLH + 25 μg Her-KLH 11.8; 7.3 0.8 50 μg CpG Fentanyl Fentanyl + Heroin
Heroin Fentanyl + Heroin
Control 50 μg KLH n/a 0.8 50 μg CpG Fentanyl Fentanyl + Heroin
Heroin Fentanyl + Heroin
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a

Antinoc. stands for antinociception; BBB stands for blood-brain barrier study. Blue corresponds to mice receiving only fentanyl conjugate vaccines; red corresponds to mice receiving only heroin conjugate vaccines; purple corresponds to mice receiving admixture of heroin and fentanyl conjugate vaccines; and white corresponds to the control group. In the admixture and control group, the 12 mice were split to test either heroin or fentanyl during an antinociception assay.