Table 1.
Vaccination Group | Immunoconjugate (μg/dose) | Conjugation Number | Alum (mg/mL) | Adjuvant | Antinoc. Drug | BBB Drug |
---|---|---|---|---|---|---|
Fent-KLH | 50 μg Fent-KLH | 11.8 | 0.8 | 50 μg CpG | Fentanyl | Fentanyl + Heroin |
Her-KLH | 50 μg Her-KLH | 7.3 | 0.8 | 50 μg CpG | Heroin | Fentanyl + Heroin |
Fent-KLH + Her-KLH | 25 μg Fent-KLH + 25 μg Her-KLH | 11.8; 7.3 | 0.8 | 50 μg CpG | Fentanyl | Fentanyl + Heroin |
Heroin | Fentanyl + Heroin | |||||
Control | 50 μg KLH | n/a | 0.8 | 50 μg CpG | Fentanyl | Fentanyl + Heroin |
Heroin | Fentanyl + Heroin | |||||
Antinoc. stands for antinociception; BBB stands for blood-brain barrier study. Blue corresponds to mice receiving only fentanyl conjugate vaccines; red corresponds to mice receiving only heroin conjugate vaccines; purple corresponds to mice receiving admixture of heroin and fentanyl conjugate vaccines; and white corresponds to the control group. In the admixture and control group, the 12 mice were split to test either heroin or fentanyl during an antinociception assay.