Skip to main content
. Author manuscript; available in PMC: 2019 Jun 19.
Published in final edited form as: Immunity. 2018 Jun 5;48(6):1258–1270.e6. doi: 10.1016/j.immuni.2018.04.015

Figure 6.

Figure 6

Fetal thymic organ cultures allow for maturation of thymic cell populations. (A) Schematic of organ culture model assessment. (B) Cell atlas-based classification of FTOC cell populations. (D) Display of thymocyte maturation (Il2ra versus Cd44) in cell atlas compared to FTOC endpoint via TACS (upper panel) and unbiased fractional identity estimation (lower panel). (E) Display of TEC maturation (Epcam versus MHCII) in cell atlas compared to FTOC endpoint via TACS (upper panel) and unbiased measurement of epithelial cell maturation using a fractional identity estimation method (lower panel). Tests compare WT E13.5 samples to the FTOC, corresponding to a null hypothesis of no maturation after dissection. P-values were computed by Fisher’s exact test with cells and grouped by TACS quadrant. Tests involve 3664 T cells and 2623 TECs. FTOC samples represent n=3 biological replicates and 3 independent experiments. See also Fig. S6 and S7.