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. 2018 Jun 21;8:9482. doi: 10.1038/s41598-018-27210-w

Figure 3.

Figure 3

Increased p65 phosphorylation and mRNA expression of NF-κB target genes in co-cultured MDA-MB-231 cells. MDA-MB-231 breast cancer cells were co-cultured either with 3T3-L1 adipocytes (CoAd), 3T3-L1 fibroblasts (CoFi), Adipocyte-conditioned medium (ACM) or alone for 5 days. (a) mRNA expression levels of selected NF-κB-response genes were analysed by qRT-PCR analyses. Data are presented as the means ± SD of triplicates from one representative experiment. Significance is relative to control unless indicated otherwise (*p < 0.05; **p < 0.005; ***p < 0.0005). (b) Representative immunoblots of indicated whole cell extracts of MDA-MB-231 cells probed with antibodies against NF- κB p65, phosphorylated-NF- κB p65 and Gapdh (loading control). Blots’ images where cropped to show relevant areas.