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. 2018 Jun 21;8:9481. doi: 10.1038/s41598-018-25994-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PMCs were infected by lentiviruses carrying either shILK (ILK) or an empty vector (EV) control. PMCs were overlaid with fibrin via incubation with a fibrinogen and thrombin mixture for 0, 1 and 2 hr. Expression of transduction factors were evaluated in triplicate by Western blotting. The relative expression of (A) ILK vs. β-actin, (B) p-Akt vs. Akt, (C) p-GSK-3β vs. GSK-3β, (D) p-P65 vs. P65 and (E) IκBα vs. β-actin are presented as a percentage of increase over untreated PMCs (NT). *P < 0.05 vs. NT; ^#P < 0.05 vs. EV at the same incubation time.