Fig. 2.

A CD44/Zeb1 loop marks IC. a Expression of Zeb1 is low in normal lung, AAH, and AD at P170. b CD44 is likewise low in normal lung, AAH, and AD at P170. c, c′ Zeb1 is induced and E-cad is repressed in cell clusters. d, d′ CD44 and Vimentin are induced along with Zeb1 in cell clusters. Arrows indicate the same position. e, e′ Zeb1 and CD44 are co-expressed in cell clusters. Arrows indicate the same position. f In cell clusters, CD44 is expressed on IC but not AD or cancer cells (AC). g In situ hybridization showing miR-200 is low in normal lung, AAH, and AD. h miR-200 is repressed in clusters. Arrows show the border of the cluster. i Bmi1 is low in normal lung, AAH, and AD. j Bmi1 is induced in clusters. k Real-time PCR showing heterozygous mutation of Zeb1 in mouse embryo fibroblasts (MEFs) is sufficient for induction of miR-200, and repression of CD44 and Bmi1 mRNAs in cell culture. Bars are 50 μm