Fig. 8.
A threshold level of Zeb1 is required for CGC formation and cancer cell generation. a Quantification of the effects of heterozygous mutation of Zeb1 on CGC at P170. EF5+ and EF5++ sites are described in Fig. 1d, e, k. CGC and AC cells in clusters were identified by nuclear morphology and expression patterns. CGC = CD44hi; AC = Zeb2−, E-cad+. b Heterozygous mutation of Zeb1 does not affect AD formation, but it inhibits AC. c, d Zeb1 shRNA or control lentivirus was delivered intratracheally biweekly beginning at P30 and ending at P90. Then, lungs were examined at P170. Panel c shows efficient infection of the lung assessed by GFP expressed from the virus. Quantification of the effect of the Zeb1 shRNA on EF5++ cluster formation and generation of AC cells in the EF5+ regions of AD is shown. EF5++ clusters and AC cells observed were GFP−, indicating they had arisen from uninfected cells. The bar is 50 μm