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. 2018 Jun 21;8:9427. doi: 10.1038/s41598-018-27409-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro epithelial-to-mesenchymal transition (EMT) induced by Matrigel culture in inflammatory breast cancer. (A) Western blots for E-cadherin, vimentin, and β-actin (a loading control) in a panel of breast cancer cells. ER+, estrogen receptor-positive; TNBC, triple-negative breast cancer; IBC, inflammatory breast cancer. SUM149 is an IBC cell line with triple-negative characteristics. Full-length blots with different exposure times are presented in Supplementary Figures S1 and S2. (B) Bright-field images depicting typical morphologies of breast cancer cells cultured in monolayer or Matrigel culture conditions. The left 2 columns and the right column are the images captured by objective lens with 10X and 4X magnifications, respectively. (C) Transcriptional profiling of EMT markers in IBC cells cultured in monolayer or Matrigel culture conditions, assayed by quantitative RT-PCR. For each marker, the samples from monolayer culture conditions were set as normalizers, and all relative expression values were log2 transformed. Bars, standard error of the mean. (D) Immunofluorescent images depicting expression of E-cadherin (green) and vimentin (red) in SUM149 cells cultured in monolayer or Matrigel culture conditions. Merged images are derived from the images of E-cadherin, vimentin, as well as nuclear counterstaining Hoechst 33342 (blue). Bar, 200 µm.