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. 2018 Jun 21;8:9427. doi: 10.1038/s41598-018-27409-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differentially expressed genes in Matrigel-cultured SUM149 cells represent EMT and inflammatory response pathways. (A) Heatmap of transcripts from monolayer- and Matrigel-cultured SUM149 cells (a triplicate sample from each) showing the 152 differentially expressed probe sets between these two conditions. Orange and blue indicate higher and lower relative expression, respectively. (B) Heatmap of EMT-related transcripts from monolayer- and Matrigel-cultured SUM149 cells (a triplicate sample from each). (C,D) Canonical pathway analyses by Ingenuity pathway analysis (IPA) software of overexpressed (C) and underexpressed (D) genes in Matrigel-cultured SUM149 cells compared to monolayer-cultured SUM149 cells. Annotated pathways were ranked according to the values of −log (P-value).