Figure 3.

Gene signature derived from Matrigel-cultured SUM149 cells represents activated inflammatory response genes in human TNBC. (A,B) Heatmaps of Wang (A) and MDA233 (B) breast cancer data sets with overexpressed genes in Matrigel-cultured SUM149 cells. Unsupervised hierarchical clustering was applied to draw these heatmaps. Before the clustering, patients in each data set were categorized into 3 subgroups: ER+ HER2−, HER2+, and TNBC, according to the transcriptional levels of ESR1 and ERBB2 (or HER2) as previously described⁴⁰. Red boxes denote gene clusters that were highly correlated with TNBC sample clusters. Orange and blue indicate higher and lower relative expression, respectively.