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. 2018 Jun 21;8:9427. doi: 10.1038/s41598-018-27409-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Colony stimulating factor 1 (CSF-1) is associated with in vitro EMT in IBC cells. (A) Transcriptional profiling of inflammation-related genes in SUM149 and SUM190 IBC cells cultured in monolayer and Matrigel culture conditions, assayed by quantitative RT-PCR. For each marker, the samples from monolayer culture conditions were set as normalizers, and all relative expression values were log2 transformed. Bars, standard error of the mean. (B) Bright-field images depicting typical morphologies of SUM149 and SUM190 cells cultured in Matrigel culture conditions treated with different concentrations of a CSF-1 receptor inhibitor, BLZ945. (C) Transcriptional profiling of EMT markers in Matrigel-cultured SUM149 cells treated with different concentrations of BLZ945, assayed by quantitative RT-PCR. For each marker, the samples from untreated controls were set as normalizers, and all relative expression values were log2 transformed. Bars, standard error of the mean. (D) Western blots for CSF-1 and E-cadherin in SUM149 cells cultured in monolayer or Matrigel, in which SUM149 cells were treated with BLZ945 (0, 2, or 5 µM in the final concentration). The bands (at around 48 kDa) stained with Ponceau S are shown as a loading control. Full-length blots with different exposure times are presented in Suplementary Fig. S11.