Fig. 7.

Studies on the storage stability of different types of enzyme preparations a PpATaseWT and b PbATaseWT. The conversions were measured after 0 days (black columns), 14 days (dark gray columns), 35 days (pale gray column), and 63 days (white column) after preparing the catalyst. Assay conditions: Cell preparation of the respective recombinant ATase (20 mg or vol. ≡ 20 mg lyophilisate), KPi-buffer (50 mM, pH 7.5), resorcinol (8, 10 mM), DAPG (15 mM), DMSO (10 vol%), 35 °C, 30 min, 750 rpm; LyoCells, (lyophilized cells, KPi-buffer, 4 °C); LyoCells/Ar, (lyophilized cells, inert storage, KPi-buffer, 4 °C); LyoCells/PBS (Lyophilized cells, PBS, 50 Mm, pH 7.5, 4 °C), LyoCFE, (lyophilized cell-free extract, KPi-buffer, 4 °C); LyoCFE/Ar, (lyophilized cell-free extract, inert storage, KPi-buffer, 4 °C); CFE/4 °C, (liquid cell-free extract, KPi-buffer, 4 °C); CFE/− 20 °C, (liquid cell-free extract, KPi-buffer, − 20 °C); CFE/− 80 °C, (liquid cell-free extract, KPi-buffer, − 80 °C)