Fig. 3.

Hyperplastic renin cells in AS^−/− mice express EP2 and EP4 receptors. In situ hybridization with the RNAscope method showed clear expression of both EP2 receptor mRNA (A; blue dots) and EP4 receptor mRNA (B; blue dots) in hyperplastic renin cells of AS^−/− mice. Hyperplastic renin cells (thin dotted line) were identified as PDGFR-β mRNA-expressing (red dots) granular cells, surrounding afferent arterioles (aa). G and thick dotted lines indicate glomeruli. Bars indicate 50 μm