Fig. 2.

Schematic diagram showing transvitreal grafting of retinal tissue derived from hPSCs in subretinal space. (a) human pluripotent stem cell differentiated into retinal lineage. (b) Three dimensional retinal organoids growing in a dish. (c) Phase contrast image of retinal organoids showing different layers differentiating within retinal organoid. (a) hESCs derived retinal organoid coimmunostained for RPE marker ZO-1 and human nuclei marker HNu. (b) Retinal organoid coimmunostained for multipotential retinal progenitor markers OTX2 and PAX6. DAPI stained nuclei. d. A piece of retinal tissue derived from hESCs is transplanted transvitreally into subretinal space