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. 2018 May 17;33(3):213–226. doi: 10.1007/s12250-018-0030-5

Fig. 7.

Fig. 7

IFN-γ-secreting memory splenocytes in immunized mice. All the mice were euthanized at week 6 (2 weeks after the last immunization), and the spleens were collected and assayed. Splenocytes were isolated and stimulated with 10 μg purified ZIKV VLPs (VLP) or 5 μg purified inactive ZIKV (PIV). IFN-γ-secreting cells were identified by ELISpots after stimulation. All data are presented as the mean ± SD of six mice in each group. The results of two independent experiments (with technical duplicates for each sample) are presented. *P < 0.05, **P < 0.01; ***P < 0.001.