Fig. 7.

IFN-γ-secreting memory splenocytes in immunized mice. All the mice were euthanized at week 6 (2 weeks after the last immunization), and the spleens were collected and assayed. Splenocytes were isolated and stimulated with 10 μg purified ZIKV VLPs (VLP) or 5 μg purified inactive ZIKV (PIV). IFN-γ-secreting cells were identified by ELISpots after stimulation. All data are presented as the mean ± SD of six mice in each group. The results of two independent experiments (with technical duplicates for each sample) are presented. *P < 0.05, **P < 0.01; ***P < 0.001.