Table 1.
Immune responses elicited by the VLP vaccine and controls
| VLP | PIV | NC | PBS | |
|---|---|---|---|---|
| Dose (μg) | 50 | 5 | 50 | – |
| NAb (log10) | 1.8 | 2.9 | 0.9 | 0.9 |
| IgG (log10) | 4.7/2.3 | 2.9/3.7 | 3.3/1.6 | 1.4/1.4 |
| IFN-γ (spots) | 928/318 | 702/803 | 263/89 | 59/30 |
| IFN-γ (pg/mL) | 161.4/50.0 | 58.2/97.0 | 18.4/22.6 | 5.7/7.3 |
| IL-2 (pg/mL) | 403.9/93.5 | 285.2/191.7 | 98.8/15.1 | 9.8/7.0 |
| IL-4 (pg/mL) | 31.7/13.7 | 23.4/29.0 | 13.7/3.9 | 3.5/3.1 |
| IL-10 (pg/mL) | 1915.1/1085.5 | 668.7/714.0 | 268.2/121.7 | 59.2/36.9 |
VLP, ZIKV VLP vaccine; PIV, purified inactivated vaccine; NC, negative control (vAc-hsp70-egfp-infected Sf9 cell lysates); PBS, blank control. NAb and IgG show the geometric mean titers of neutralization and virus-specific titers in serum collected at week 6. For IgG titers, the left values represent the VLP-specific IgG titers and the right values represent ZIKV-specific IgG titers. IFN-γ (spots) indicates the number of IFN-γ-secreting splenocytes per 1 × 106 cells. For IFN-γ, IL-2, IL4 and IL-10, the left and right values represent the production stimulated by VLPs and inactivated ZIKV, respectively. n = 6 for all groups.