Figure 3.

Expression analysis of bagA. Northern blot analysis of wild type (RJMP103.5) in response to apoptotic stress (2 mM Farnesol) and oxidative stress (2 mM H₂O₂) is shown. Ribosomal RNA (rRNA) was used as a loading control (A). Cultures were harvested either 30 min or 1 h after adding the stressors and blots were probed with bagA, the bands were quantified in ImageJ and plotted for significance in Graphpad prism (B). Strains were grown under sexual development inducing conditions and harvested at specified time intervals. Ribosomal RNA (rRNA) was used as a loading control. Duplicates of each time point are shown (C), the bands were quantified in ImageJ and plotted for significance in Graphpad prism (D). Stars (^*) indicate statistical significance (p < 0.05).