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. 2018 Jun 15;9:631. doi: 10.3389/fphar.2018.00631

Figure 6.

Figure 6

Immuno-blot analyses for pCREB and BDNF expression in the ileum and colon. Mice were exposed to the stress (subjected to CACS) and daily treated with vehicle (Veh), trans-Resveratrol (Resv, 2.5, 5.0, and 10.0 mg/kg, i.g.), rolipram (Rol, 1.25 mg/kg, i.p.), desipramine (Des, 0.5 mg/kg, i.p.) or diazepam (Dia, 10 mg/kg, i.p.) 30 min before stress for 3 weeks. (A) Photomicrographs of representative immune-blotting bands; (B–D) PDE4A expression, the ratio of pCREB to total CREB and BDNF protein level in the ileum were measured. (E) Photomicrographs of representative immune-blotting bands. (F–H) PDE4A, the ratio of pCREB to total CREB and BDNF levels were measured in the colon. Results are expressed as mean ± SEM (n = 8). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. Ctrl group, #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. CACS + Veh group.