Table 1.

Chronic-acute combined stress (CACS) procedure.

Day	Stress
1	Cold environment (4°C, 15 min) and food deprivation (4 h)
2	Cage tilting and water deprivation (4 h)
3	Bedding damp (2 h) and cold swim (12°C, 5 min)
4	Restraint (2 h) and tail pinch (5 min)
5	Food & water deprivation (6 h) and foot shock (3 min; 36 V)
6	Cold swim (12°C, 10 min) and overnight illumination (12 h)
7	Bedding damp (4 h) and tail pinch (10 min)
8	Restraint (3 h) and cold swim (12°C, 5 min)
9	Tail pinch (15 min) and overnight illumination (12 h)
10	Bedding damp (6 h) and foot shock (6 min; 36 V)
11	Restraint (4 h) and overnight illumination (12 h)
12	Cold swim (12°C, 10 min)
13	Overnight bedding damp (12 h) and tail pinch (20 min)
14	Restraint (6 h)
15	Cage tilting (6 h) and foot shock (10 min; 36 V)
16	Cold swim (12°C, 10 min) Food & water deprivation (6 h)
17	Overnight bedding damp (12 h) and tail pinch (30 min)
18	Cold environment (4°C, 20 min) and overnight illumination (12 h)
19	Restraint (6 h)