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. 2018 Jun 15;9:1253. doi: 10.3389/fmicb.2018.01253

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Copyright © 2018 Mueller, Hantsch, Volkmer and Staege.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Epigenetic regulation of HERVK and PRODH in GCT cells. Presented are results from quantitative RT-PCR analysis with primers specific for HERVK and PRODH. cDNA was prepared from GCT cell lines and β-actin was used as house-keeping control. Expression of HERVK in un-treated cells was set as 1 and relative expression was calculated according to the 2^-ΔΔCt-method (Livak and Schmittgen, 2001). (A) Pluripotent H12.1 cells were treated with retinoic acid to induce differentiation. (B) Somatically differentiated 1777NRpmet cells were treated with a combination of 5′-azacytidine and trichostatin A to induce re-expression of HERVK and PRODH.