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. 2018 Jun 15;9:420. doi: 10.3389/fneur.2018.00420

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Copyright © 2018 Tully, Tang, Zheng, Acosta, Tian, Hayward, Race, Mattson and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of acrolein changes in spinal cord tissue by immunoblotting in EAE mice. The acrolein-lysine adducts in control and in EAE were detected using Bio-Dot SF microfiltration apparatus. Band intensities were analyzed using image J (NIH) and expressed in arbitrary units. Note the scatter plot demonstrated an increase of acrolein-lysine adducts in EAE. (^*P < 0.01 when compared to control, t-test). N = 9 in each group of acrolein measurement in tissue.