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. 2018 Jun 15;8:182. doi: 10.3389/fcimb.2018.00182

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Copyright © 2018 Champion, Bandara, Mohapatra, Fulton, Twine and Inzana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot and SDS-PAGE silver stain of CLC. (A) Hyperimmune (HI) rabbit serum to CLC extracted with phenol and reacted to CLC extracted with phenol (P) or urea (U); (B) HI rabbit serum to phenol-extracted CLC adsorbed with CLC-deficient strain WbtI_G191VΔ1423-22_P10 and reacted with phenol (P)—or urea (U)-extracted CLC. (C) Silver stain of CLC extracted with 0.5% phenol (P) or 1 M urea (U). Similar proteins were evident in both the phenol- and the urea-extracted CLC, but a HMS band was more prominent in the urea-extracted CLC. After the CLC HI sera was adsorbed with the CLC-deficient mutant only one band/doublet was evident at about 45-kDa, which is the same size as that fractionated with the GelFree 8100 (Figure 2).