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CLC fractionation using Triton X-114 and sarkosyl detergents. 4–12% NuPAGE SDS-PAGE stained using StainsAll/silver. Lanes: 1 and 5, TxS-A; 2 and 6, TxS-D; 3 and 7, TxI-SS; 4 and 8, TxI-SI; 9,) non-fractionated 1 M urea extract of CLC. CLC from WbtIG191V_P10 fractioned predominantly into the TxS-A phase and the TxI-SS phase.
