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. 2018 Jun 15;8:182. doi: 10.3389/fcimb.2018.00182

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Copyright © 2018 Champion, Bandara, Mohapatra, Fulton, Twine and Inzana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron microscopy of OMV/T_A purified from F. tularensis strains grown on agar. OMV/T_A were isolated from LVS_P10, LVSΔ1423-22_P10, WbtI_G191V_P10, or WbtI_G191VΔ1423-22_P10 grown on CDM agar following a modified protocol (Gamazo et al., 1989; Avila-Calderón et al., 2012). (A,B) LVS_P10 (50 k magnification and 120 k magnification, respectively); (C,D) LVSΔ1423-22_P10 (50 k magnification and 120 k magnification, respectively); (E,F) WbtI_G191V_P10 (50 k magnification and 120 k magnification); (G,H) WbtI_G191VΔ1423-22_P10 (50 k magnification and 120 k magnification, respectively). Red arrows, OMT_A (along black lines); blue arrows, OMV_A; yellow circles, non-specified material between the OMV/T_A.