Figure 9.

Protective efficacy of CLC-conjugate in BALB/c mice challenged intradermally and intranasally, and cytokine response pre- and post-challenge. (A) Survival of BALB/c mice challenged ID or IN with 5 × LD₅₀ of LVS after ID immunization with 50 μg of KLH- or flagellin-conjugated CLC. Mice immunized with either conjugate were significantly protected against ID challenge, and mice immunized with KLH-CLC were protected against IN challenge (^*p < 0.05). (B) SDS-PAGE of conjugated KLH-CLC and unconjugated KLH, showing the increase in size of the conjugate. (C) Pre-immunization serum cytokine response of all mice; (D) cytokine response from sera of mice collected 2 weeks after the final immunization with KLH-CLC or flagellin-CLC; (E) cytokine response from sera collected 3 days post-challenge.