Table 4.
Proteins identified in the insoluble fraction of CLC from LVS.
| Identifier | Proteins previously reported, novel, or among 20 most common (T20): | Description | Protein_score | Mass (Da) | Peptide_expect |
|---|---|---|---|---|---|
| Insoluble | |||||
| FTL_1751 | E, S | Elongation factor Tu (EF-Tu) | 969 | 43,363 | 2.00E-05 |
| FTL_1714 | T20, P, C | Chaperone protein, groEL | 802 | 57,367 | 2.90E-06 |
| FTL_1191 | P, C | Chaperone protein dnaK (heat shock protein family 70 protein) | 394 | 69,140 | 1.10E-07 |
| FTL_1907 | E, S | Cell division protein | 381 | 39,720 | 3.10E-08 |
| FTL_0113 | N, P | Intracellular growth locus, subunit C | 368 | 22,119 | 1.30E-05 |
| FTL_0234 | E, S | Elongation factor G (EF-G) | 250 | 77,681 | 2.00E-05 |
| FTL_0112 | P, S | Intracellular growth locus, subunit B | 226 | 57,881 | 2.50E-13 |
| FTL_1795 | E, S | ATP synthase beta chain | 224 | 49,834 | 3.40E-07 |
| FTL_0891 | N | Trigger factor (TF) protein (peptidyl-prolyl cis/trans isomerase) | 190 | 49540 | 1.70E-10 |
| FTL_0094 | P, E, S | ClpB protein | 178 | 95,987 | 1.00E-07 |
| FTL_0225 | N | Protein chain elongation factor EF-Ts | 177 | 30,940 | 1.40E-06 |
| FTL_1146 | T20, C | Glyceraldehyde-3-phosphate dehydrogenase | 161 | 35,391 | 0.0003 |
| FTL_0267 | P, S | Chaperone Hsp90, heat shock protein HtpG | 151 | 72,326 | 1.00E-07 |
| FTL_1791 | N, C | Superoxide dismutase | 148 | 21,926 | 9.30E-07 |
| FTL_1912 | E, S | 30S ribosomal protein S1 | 145 | 61,631 | 0.00017 |
| FTL_1461 | N, C | Purine nucleoside phosphorylase | 132 | 26,848 | 0.0007 |
| FTL_0436 | N | Isoleucyl-tRNA synthetase | 127 | 106,904 | 6.00E-13 |
| FTL_0572 | E, S | Hypothetical protein | 113 | 51,945 | 1.10E-11 |
| FTL_0260 | S | 30S ribosomal protein S4 | 107 | 23,222 | 8.10E-11 |
| FTL_0597 | N | NAD dependent epimerase | 105 | 36,427 | 1.80E-07 |
| FTL_0895 | N, C | Histone-like protein HU form B | 105 | 9,468 | 1.20E-10 |
| FTL_1190 | N | Chaperone protein grpE (heat shock protein family 70 cofactor) | 102 | 22,022 | 2.50E-06 |
| FTL_0166 | S | Universal stress protein | 102 | 30,202 | 2.10E-06 |
| FTL_1746 | S | 50S ribosomal protein L10 | 101 | 18,720 | 1.90E-10 |
| FTL_0949 | N | Ribose-phosphate pyrophosphokinase | 97 | 34,887 | 2.70E-06 |
| FTL_0987 | S | Lactate dehydrogenase | 94 | 34,055 | 5.90E-07 |
| FTL_0588 | N | Isocitrate dehydrogenase | 93 | 82,332 | 7.70E-05 |
| FTL_0834 | S | Rhodanese-like family protein | 88 | 27,847 | 4.90E-09 |
| FTL_1553 | P, S | Succinyl-CoA synthetase beta chain | 87 | 41,516 | 1.30E-06 |
| FTL_1527 | S | Enolase (2-phosphoglycerate dehydratase) | 85 | 49,480 | 4.30E-09 |
| FTL_1701 | P | GlpX protein | 83 | 34,820 | 1.90E-08 |
| FTL_0923 | S | Glutaredoxin 2 | 83 | 25,135 | 1.30E-08 |
| FTL_1224 | N | Thioredoxin 1 | 78 | 12,194 | 6.60E-08 |
| FTL_1743 | T20,P, S,E | DNA-directed RNA polymerase, β subunit | 77 | 157,289 | 6.20E-08 |
| FTL_1591 | S | Acetyl-CoA carboxylase, biotin carboxylase subunit | 76 | 50,019 | 7.50E-08 |
| FTL_0097 | N | Hypothetical protein | 76 | 13,785 | 6.10E-05 |
| FTL_1780 | N, C | Triosephosphate isomerase | 76 | 27,638 | 5.80E-08 |
| FTL_0232 | S | 30S ribosomal protein S12 | 69 | 13,806 | 9.70E-07 |
| FTL_1350 | N | Glycyl-tRNA synthetase beta subunit | 66 | 77,973 | 4.80E-07 |
| FTL_1140 | N | Malonyl coA-acyl carrier protein transacylase | 62 | 33,480 | 2.30E-06 |
| FTL_0233 | S | 30S ribosomal protein S7 | 61 | 17,796 | 2.60E-06 |
| FTL_1138 | N | acyl carrier protein | 61 | 10,653 | 2.30E-06 |
| FTL_1240 | S | Phospho-2-dehydro-3-deoxyheptonate aldolase | 60 | 40,853 | 1.80E-06 |
| FTL_1747 | S | 50S ribosomal protein L1 | 57 | 24,626 | 4.70E-06 |
| FTL_1275 | N | Dethiobiotin synthetase | 55 | 24,487 | 9.70E-06 |
| FTL_1592 | T20 | Acetyl-CoA carboxylase, biotin carboxyl carrier protein subunit | 53 | 16,394 | 1.60E-05 |
| FTL_1334 | N | L-serine dehydratase 1 | 52 | 49,977 | 2.70E-05 |
| FTL_0387 | N | Aspartate transaminase | 52 | 44,355 | 2.60E-05 |
| FTL_1442 | T20, S | Enoyl-[acyl-carrier-protein] reductase (NADH) | 49 | 27,757 | 4.10E-05 |
| FTL_0916 | E, S | Ketol-acid reductoisomerase | 46 | 37,855 | 7.30E-05 |
| FTL_1797 | N | ATP synthase alpha chain | 46 | 55,502 | 5.20E-05 |
| FTL_0311 | S | Dihydrolipoamide dehydrogenase | 44 | 50,495 | 9.90E-05 |
| FTL_1772 | T20, S | Aconitate hydratase | 44 | 102,639 | 0.00028 |
| FTL_1784 | E | 2-oxoglutarate dehydrogenase E1 component | 39 | 105,613 | 0.00029 |
| FTL_0739 | N | Glucose inhibited division protein A | 36 | 69,721 | 0.00053 |
| FTL_1022 | N | Coproporphyinogen III oxidase | 36 | 35,854 | 0.00053 |
T20, Top 20 proteins found in F. novicida OMV (Pierson et al., 2011); P, Proteins previously associated with pathogenesis (Pechous et al., 2008); E, Proteins found in exponential phase OMV/T (McCaig et al., 2013); S, Proteins found in early stationary phase OMV/T (McCaig et al., 2013); C, Proteins found in culture filtrate (Cervenka et al., 2010). N = Novel to this work.