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. 2018 Jun 15;9:699. doi: 10.3389/fphys.2018.00699

FIGURE 1.

FIGURE 1

Bioassay guided fractionation of cardioprotective components from ginseng extract. (A) The TLC spectrum of the 50 fractions of ginseng extract in HSCCC separation. The TLC spectrum of fraction 1–17 of ginseng extract (1); The TLC spectrum of fraction 18–34 of ginseng extract (2); The TLC spectrum of fraction 35–50 of ginseng extract (3). (B) Molecular weight distribution of ginseng extracts 2 and 3. (C) Protective effects of ginseng extracts in H9c2 cells exposed to H/R injury. (D) Protective effects of fractions of ginseng extract 2/3 in H9c2 cells exposed to H/R injury. (E) Protective effects of five fractions of total 70% ethanol precipitates fraction in H9c2 cells exposed to H/R injury. (F) Molecular weight distribution of AP1. (G) Cytotoxicity of AP1 in H9c2 cells under normoxic conditions. MVS assay was employed to measure cell viability. The results are presented as a percentage of normal group. Data are presented in mean ± SD of three independent experiments. N = 3. (C–E) ˆ ˆ ˆ P < 0.001 vs. Normal, ∗∗P < 0.01 vs. H/R, ∗∗∗P < 0.001 vs. H/R, (G) ∗∗∗P < 0.001 vs. Normal.