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. 2018 Jun 15;8:196. doi: 10.3389/fcimb.2018.00196

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Copyright © 2018 Alves, Booth, Scavone, Schellenberger, Salvage, Dedi, Thet, Jenkins, Waters, Ng, Overall, Metcalfe, Nzakizwanayo and Jones.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of simulated burn wounds. To confirm that the BWAD was able to generate representative thermal injuries in ex-vivo porcine skin sections, areas of skin in contact with the BWAD after heating to 100°C were examined by histology and directly by environmental scanning electron microscopy (ESEM), and compared to uninjured skin. (A) Thin sections of undamaged and burned skin subjected to H&E staining and histological analysis (x100 magnification). The stratum corneum (SC), Epidermis (E), and dermis (D) are clearly visible and discernible on the undamaged skin sections. In contrast, these structures are disrupted in skin section from BWAD exposed skin, consistent with partial thickness burn injuries. (B) ESEM of the surface of undamaged porcine skin and areas in contact with the BWAD. Scale bar = 20 μm.