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Volcano plot of the proteomes of DK80 and Δcpubi4. Black dots represent the total proteins identified from both DK80 and Δcpubi4, red dots represent the differentially expressed proteins in Δcpubi4 with fold-change greater than 1.5 and p < 0.05. A total of 142 proteins exhibited reduced ubiquitination upon deletion of cpubi4, indicating that they are the substrates of CPUBI4.
