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. 2018 Jun 15;9:1282. doi: 10.3389/fimmu.2018.01282

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Copyright © 2018 Cox, Danby, Hernandez, Laza-Briviesca, Pearson, Madrigal and Saudemont.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcriptional analysis of MHC class I-related chain A (MICA) and MHC class I-related chain B (MICB) promoter regions in proliferating HCT116 cells. Normalised percent luciferase activity relative to SV40 control plasmid transfection is shown. Assay conditions were performed in triplicate with three independent preparations of plasmid construct DNA and repeated for a total of four independent experiments. (A) MICA promoter driven luciferase transcription relative to SV40 promoter in proliferating HCT116 cells 24 h post-transfection. (B) MICB promoter driven luciferase transcription relative to SV40 promoter in proliferating HCT116 cells 24 h post-transfection. Statistical analysis was performed using Mann–Whitney test ± SEM (**P < 0.01 and ***P < 0.001).