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Cell viability and PMP in C. neoformans- and C. gattii-infected BMDM. (A) The percentages of populations of live, dead, and apoptotic infected BMDM were determined. (B) Number of cells that have phagolysosomal membrane permeabilization, determined by selecting the LysoTracker Deep Red-negative population of infected cells. Three independent biological replicates were performed for each experiment (shown). No statistically significant difference was determined (two-way ANOVA).
