FIG 3.

CpxRA is dispensable for induction of S. Typhimurium colitis on day 2 postinfection. Streptomycin-treated C57BL/6 mice (n = 5) were infected intragastrically for 2 days with 5 × 10⁷ CFU S. Typhimurium wild-type strain SL1344 or ΔcpxRA::kan mutant strain T192. (A) S. Typhimurium loads in feces on day 2 postinfection. (B) Representative H&E-stained cecal sections (magnification, ×100). Bars, 100 μm. (C) Cecal pathological scores for H&E-stained cecal tissue sections. (D) Fecal lipocalin-2 levels monitored by an ELISA. Data points represent means ± standard deviations. For panels A and C, bars indicate the medians. ns, not significant (P ≥ 0.05) (as determined by a Mann-Whitney U test).